Genomics

Workshop: Genomic Approaches in Biotechnology

June 23-27, 2014 • 9:00 AM to 5:00 PM • Universidad del Turabo • Gurabo, PR

Instructors: Dr. José Pérez-Jiménez (Universidad del Turabo, PR) 

Dr. Thomas Tubon (Madison Area Technical College, WI)

Materials developed by DNA Learning Center, Cold Spring Harbor Laboratory, New York, USA 

Central Dogma for Eukaryotes

general concept (video)

DNA Barcoding

dedicated website: http://www.dnabarcoding101.org 

history (video)
presentation (here)

webinar (click here)

general protocol (animation)

PCR: polymerase chain reaction (video, animation)

gels amplicons

C. elegans

basic introduction (video) 2:10 m at OpenWorm

extended introduction (video) 26:05 m

protocols: chunking (video by UWGSEO)

phenotypes: 

• wild type (video, video, video), 
• blister (video; video), 
• dumpy (video; video): "C. elegans dpy-3 mutant. The dpy-3 gene encodes a cuticular collagen. This mutant shows a "dumpy" phenotype." [‪SugimotoLab]
• roller (video; video): "C. elegans rol-1 mutant. The rol-1 gene encodes a cuticular collagen. This mutant shows a "roller" phenotype in which the body of the animal rotates around its long axis as the animal moves, causing the animal to move in a circular pattern." [‪SugimotoLab]
• uncoordinated (video; video). "C. elegans unc-8 mutant. The unc-8 gene encodes an amiloride-sensitive DEG/ENaC cation-selective channel subunit. This mutant shows the "coiler" phenotype in which the body of an animal assumes a coil-like shape when it attempts to move." [‪SugimotoLab]
• ‪SugimotoLab (YouTube channel)

RNAi

introduction [ppt]

mechanism [ppt]

screening tests [ppt]

DNA Subway

http://dnasubway.iplantcollaborative.org 
bioinformatic introduction [ppt]
look at background and tour for details
blue line [ppt]
manual [http://dnasubway.iplantcollaborative.org/files/pdf/DNA_Subway_Guide.pdf]